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Northern Eclipse
Help Reference |
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The Line Scan function can be used
to measure 1D gels or any application that requires the measurement of
intensity under a line (this function is used in our DNA comet assay developed
jointly by Empix Imaging and Princess Margaret Hospital). Once the Line Scan
icon is clicked, the following menu appears.
When the Use Starting Line: check
box is checked, a red and white dashed line is placed on the current image
window (it defaults to the top of the current window) and may be pulled down
the image to be used as a starting line for all side by side line scan
measurements.
To enable this starting line feature click on Define button. Use the Horizontal bullet option if the
starting line is for use in an east-west direction on the image (the Vertical bullet option for
north-south). The Subtract
Background check box option may be used if background noise or gray levels
need to be subtracted from line measurements. Clicking on Define background allows the background line to be placed
appropriately. Each pixel within the defined background line will be subtracted
from all subsequent measurement lines drawn. The Bright on Dark check
box must be checked on for measuring white objects on a dark background. The
default unchecked setting is for measuring dark objects on a light background.
To draw a line scan, start at the red line and pull a line down with the left
mouse button the required distance (Pull across if the Vertical option
is being used). A Line Scan dialog box will appear in the Line Scan Graph window.
By moving the mouse on the Line Scan Graph, and holding down the left button, areas of the
graph may be highlighted in yellow. This highlighting is used to specify the
area (or band) for Data to be logged to excel. Once an area of the Line Scan
Graph has been highlighted, only the data from that area under the graph will
be logged to excel once the Log Data
button has been clicked. If an area of the graph is not specified then all data
under the graph will be logged once the Log
Data button has been clicked. The Width adjustment control (spinner)
on the Line Scan Graph dialog box allows the width of both the background line
and the measurement line to be set to a desired pixel width. If the Width
adjustment is set to a value higher than one, all values will be averaged down
to a single line value in the horizontal or vertical direction, depending on
which direction measurements are being made in.
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